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Image Search Results
Journal: PLOS ONE
Article Title: The role of GAPDH in the selective toxicity of CNP in melanoma cells
doi: 10.1371/journal.pone.0300718
Figure Lengend Snippet: Subconfluent A375 melanoma cells were treated with either 2 mM H 2 O 2 for 15 minutes or 300 μM CNP for 4 h and 24 h, respectively. Thiol oxidation was assessed via Sulfo Biotics Redox State Monitoring Kit with subsequent Western Blotting and probing for GAPDH. SHx0 to SHx3 bands refer to the amount of linker which was bound to thiol groups of the protein. If all thiol groups were oxidized prior to the incubation with the protein linker/SHifter, no SHifter was able to bind to the oxidized protein (SHx0). Experiments were performed in triplicates (n = 3).
Article Snippet: The
Techniques: Western Blot, Incubation